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ActoFactor™ Recombinant Human Interleukin 17B

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Cat.No.
CSC-CTK0260
Description
IL-17B is a disulfide-linked homodimer of two 161 amino acid polypeptide chains. It belongs to the IL-17 family of structurally-related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. The six known members of this family, IL-17A through IL-17F, are secreted as homodimers. IL-17B is expressed by T-cells and has been shown to stimulate release of TNF-alpha and IL-1beta from cells of the monocyte lineage. Recombinant human IL-17B is a 36.6 kDa disulfide-linked homodimer of two 161 amino acid polypeptide chains.
Species
Human
Product Overview
Human IL17B expressed in E.coli
Molecular Mass
36.6 kDa
Size
CAT# CSC-CTK0260-10 (10 μg); CAT# CSC-CTK0260-50 (50 μg)
Expression System
E.coli
Purity
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
Activity
Determined by its ability to induce IL-8 in human PBMCs using a concentration range of 10-100 ng. Please note: Results may vary with PBMC donors.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized (freeze-dried), sterile powder. Lyophilized with no additives.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1 - 1,0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-17B should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
Customer Support & Price Inquiry
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