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ActoFactor™ Recombinant Human Interleukin 17A (E. coli)
- Specification
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Cat.No.
CSC-CTK0258
Description
IL-17 is a potent proinflammatory cytokine produced by activated memory T cells. There are at least six members of the IL-17 family in humans and in mice. As interleukin-17 shares properties with IL-1 and TNF-alpha, it may induce joint inflammation and bone and cartilage destruction. This cytokine is found in synovial fluids of patients with rheumatoid arthritis, and produced by rheumatoid arthritis synovium. It increases IL-6 production, induces collagen degradation and decreases collagen synthesis by synovium and cartilage and proteoglycan synthesis in cartilage. Interleukin-17 is also able to increase bone destruction and reduce its formation. Blocking of interleukin-17 with specific inhibitors provides a protective inhibition of cartilage and bone degradation. Recombinant human IL-17 produced in E. coli is a homodimeric, non-glycosylated polypeptide chain containing of 264 amino acids and a molecular mass of 30,250 Dalton.
Species
Human
Product Overview
Human IL17A expressed in E.coli
Molecular Mass
30.25 kDa
Size
CAT# CSC-CTK0258-25 (25 μg); CAT# CSC-CTK0258-125 (125 μg)
Expression System
E.coli
Purity
Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.
Activity
The ED50 as determined by the dose-dependent induction of IL-6 in Hs68 cell line and was found to be ~2 ng/ml, corresponding to a specific activity of 5 x 10^5 Units/mg.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Sterile-filtered white, lyophilized (freeze-dried) powder. Lyophilized from a concentrated (1 mg/ml) protein solution in 20 mM citrate, 0.1 M NaCl(pH 4.5).
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1 - 1,0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-17 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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