ActoFactor™ Recombinant Human Interleukin 12-p40

Specification
Recommended Products

Cat.No.

CSC-CTK0250

Description

Interleukin-12 (IL-12) is a heterodimer cytokine composed of p40 and p35 subunits. It is mainly produced by macrophages, dendritic cells, and B cells. It has multiple effects on T and natural killer (NK) cells. In particular, it appears to be a major factor for the development of cellular immunity. IL-12 stimulates the production and secretion of several cytokines, in particular IFN-γ, by NK cells and T cells, induces proliferation and enhances the cytotoxic activity within these cell populations. The p40 subunit of IL-12 interacts with the β-subunit of IL-12R and the disulfide linked p40 homodimer specifically antagonizes the activity of bioactive IL-12 in different assay systems. Recombinant human Interleukin-12 p40 is a glycosylated polypeptide chain containing 312 amino acids (fragment 23 – 328 P29460 IL12B_HUMAN) with an amino- terminal hexahistidine tag. The recombinant protein has a predicted molecular mass of 35.5 kDa, however as a result of potential glycosylation, the molecular mass could be approximately 40k Da by SDSPAGE under reducing conditions. Human recombinant IL-12-p40 expressed in Nicotiana benthamiana is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product does not contain any animal–derived components or impurities.

Species

Human

Product Overview

Human IL12B expressed in Nicotiana benthamiana

Molecular Mass

35.5 kDa

Size

CAT# CSC-CTK0250-10 (10 μg); CAT# CSC-CTK0250-50 (50 μg)

Expression System

Nicotiana benthamiana

Purity

Greater than 97% as determined by SDS-PAGE analysis.

Activity

rHu IL-12-p40 is fully biologically active when compared to standards. The biological activity of IL-12-p40 is determined by the dose dependent inhibition of IL-12 dependent IFN-gamma production (mouse splenocytes). Data not yet available.

Endotoxin Level

Less than 0.04 EU/μg.

Formulation

Lyophilized from a concentrated, sterile protein solution in 0.05 M Tris-HCl buffer (pH 7.4).

Reconstitution

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 50 μg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use. Optimal concentration should be determined for specific application and cell lines.

Storage & Stability

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

CSC-CTK0092

ActoFactor™ Recombinant Human Angiopoietin 1

INQUIRY

CSC-CTK0093

ActoFactor™ Recombinant Human Apolipoprotein A-I

INQUIRY

CSC-CTK0094

ActoFactor™ Recombinant Human Apolipoprotein E2

INQUIRY

CSC-CTK0095

ActoFactor™ Recombinant Human Apolipoprotein E3

INQUIRY