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ActoFactor™ Recombinant Human Interleukin 12-p40

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Cat.No.
CSC-CTK0250
Description
Interleukin-12 (IL-12) is a heterodimer cytokine composed of p40 and p35 subunits. It is mainly produced by macrophages, dendritic cells, and B cells. It has multiple effects on T and natural killer (NK) cells. In particular, it appears to be a major factor for the development of cellular immunity. IL-12 stimulates the production and secretion of several cytokines, in particular IFN-γ, by NK cells and T cells, induces proliferation and enhances the cytotoxic activity within these cell populations. The p40 subunit of IL-12 interacts with the β-subunit of IL-12R and the disulfide linked p40 homodimer specifically antagonizes the activity of bioactive IL-12 in different assay systems. Recombinant human Interleukin-12 p40 is a glycosylated polypeptide chain containing 312 amino acids (fragment 23 – 328 P29460 IL12B_HUMAN) with an amino- terminal hexahistidine tag. The recombinant protein has a predicted molecular mass of 35.5 kDa, however as a result of potential glycosylation, the molecular mass could be approximately 40k Da by SDSPAGE under reducing conditions. Human recombinant IL-12-p40 expressed in Nicotiana benthamiana is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product does not contain any animal–derived components or impurities.
Species
Human
Product Overview
Human IL12B expressed in Nicotiana benthamiana
Molecular Mass
35.5 kDa
Size
CAT# CSC-CTK0250-10 (10 μg); CAT# CSC-CTK0250-50 (50 μg)
Expression System
Nicotiana benthamiana
Purity
Greater than 97% as determined by SDS-PAGE analysis.
Activity
rHu IL-12-p40 is fully biologically active when compared to standards. The biological activity of IL-12-p40 is determined by the dose dependent inhibition of IL-12 dependent IFN-gamma production (mouse splenocytes). Data not yet available.
Endotoxin Level
Less than 0.04 EU/μg.
Formulation
Lyophilized from a concentrated, sterile protein solution in 0.05 M Tris-HCl buffer (pH 7.4).
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 50 μg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use. Optimal concentration should be determined for specific application and cell lines.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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