Recombinant human IL-11 produced in E. coli is a single, non-glycosylated polypeptide chain of 179 amino acids and a molecular mass of 19,256 Dalton.
Human IL11 expressed in E.coli
CAT# CSC-CTK0245-10 (10 μg); CAT# CSC-CTK0245-50 (50 μg)
Greater than 98% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
rHu IL-11 is fully biologically active when compared to a standard. The ED50, as determined by the dose-dependent stimulation of the proliferation of murine 7TD1, was found to be less then 10 ng/ml corresponding to a specific activity of 1 x 10^5 IU/mg.
Less than 1 EU/μg.
Sterile-filtered white, lyophilized (freeze-dried) powder. Lyophilized from a concentrated (1 mg/ml) solution in water containing no additives. Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1 - 1,0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-11 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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