Interleukin 11 (IL-11) is a pleiotropic cytokine that exerts its effects on myelopoiesis and lymphopoiesis. It can stimulate the T cell-dependent development of specific immunoglobulin-secreting B cells. IL-11 can act synergistically with IL-3, IL-4 and SCF in vitro to shorten the G0 period of early hematopoietic
progenitors. It stimulates erythropoiesis in the presence of IL-3 or SCF and enhances IL-3-dependent megakaryocyte colony formation. IL-11 promotes the development of other cell types, including adipocytes and osteoclasts, and stimulates the synthesis of hepatic acute-phase proteins. Recombinant human IL-11 contains 178 amino acids and has a molecular mass of approximately 19 kDa. It migrates in SDS-PAGE with an apparent molecular mass of 23 kDa.
Human IL11 expressed in Sf21 insect cells
A DNA sequence encoding the 199 amino acid residue human IL-11 precursor was expressed in Sf21 insect cells using a baculovirus expression system.
CAT# CSC-CTK0085-5 (5 μg); CAT# CSC-CTK0085-25 (25 μg)
Sf21 insect cells
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
The biological activity of recombinant human IL-11 is measured in a cell proliferation assay using T11, a subline of the IL-6-dependent murine plasmacytoma cell line T1165 that has been adapted to grow in IL-11. The ED50 for this effect is typically 0.06 - 0.24 ng/mL.
Endotoxin level is <1.0 EU per 1 μg cytokine, as determined by the LAL method.
Recombinant human IL-11 is lyophilized from a 0.2 μm filtered solution in phosphate buffered saline (PBS) containing 50 μg bovine serum albumin per 1 μg cytokine.
Reconstitute human IL-11 at a concentration greater than 10 μg/mL with sterile PBS containing at least 0.1% human or bovine serum albumin.
Storage & Stability
Lyophilized human IL-11 is stable for up to twelve months from date of receipt at -20°C to -70°C. Reconstituted human IL-11 can be stored under sterile conditions at 2°C - 8°Cfor one month, or at -20°C to -70°C (in a manual defrost freezer)for three months without detectable loss of activity. Avoid repeated freezing and thawing.
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