Human Interferon-alpha 2a is an interferon class I produced by the cells of the innate immune system as a part of the defense response to foreign agents such as viruses, parasites and tumor cells. The human alpha-interferon family displays broad spectrum antiviral, antiproliferative and immunomodulatory activities on a variety of cell types. Interferon-alpha 2a (IFNa-2a), one of the subtypes, is a protein consisting of 165 amino acids. Its three-dimensional loop structure is maintained by two disulfide bridges. It has an approximate molecular weight of 20 kDa. Recombinant human IFN-alpha 2a is a polypeptide single chain containing 171 amino acids with a predicted molecular mass of 20.1 kDa. It contains a His-tag at the C-terminal end. Human recombinant IFN-alpha 2a expressed in Nicotiana benthamiana is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product does not contain any animal–derived components or impurities.
Human IFNA2 expressed in Nicotiana benthamiana
CAT# CSC-CTK0179-10 (10 μg); CAT# CSC-CTK0179-50 (50 μg)
Greater than 97% as determined by SDS-PAGE analysis.
The specific activity was determined by a quantitative gene reported bioassay using human Type I interferon-sensitive cells. The activity is <8 x 10^8 units/mg in reference to a viral resistance assay using bovine kidney MDBK cells.
Less than 0.04 EU/μg.
Lyophilized from a concentrated, sterile protein solution in 0.05 M Tris-HCl buffer (pH 7.4) with 0.01% SDS.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 50 μg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use. At higher concentrations the solubility may be reduced and multimers generated. Optimal concentration should be determined for specific application and cell lines.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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