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ActoFactor™ Recombinant Human Interferon, lambda 1 (HEK)

  • Specification
  • Recommended Products
IFN lambda-1,-2, and -3 (also known as IL-29, IL-28A and IL-28B respectively) are distantly related to the IL-10 family and Interferons. All three IFN-lambdas use a distinct receptor system composed of an IFN-lambdaR1 subunit (also called CRF2-12) and IL-10R2 subunit (also called CRF2-14). Signaling through this receptor system induces antiviral defense similar but distinct than the type I Interferons. (Kotenko, SV. et al. Nat Immunology 2003 [1] p. 69-77). Recombinant human IFN-lambda 1 produced in HEK cells is a glycosylated monomer, having a molecular weight range of 29-35 kDa due to glycosylation. It has been purified by proprietary chromatographic techniques.
Product Overview
Human IFNL1 expressed in HEK cells
Molecular Mass
CAT# CSC-CTK0193-10 (10 μg); CAT# CSC-CTK0193-50 (50 μg)
Expression System
HEK cells
Greater than 95% as determined by SDS-PAGE analysis.
rHu IFN-lambda 1 is fully biologically active when compared to a standard. The specific activity was determined by the dose-dependent protection of the cytopathic effect on A549 cells (human lung adenocarcinoma epithelial cell line) that were challenged with encephalomyocarditis (EMC) virus and is typically 0.5-5 ng/ml.
Endotoxin Level
Less than 1 EU/μg.
Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized from a concentrated protein solution (1 mg/ml) in 1xPBS.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IFN-lambda 1 should be used immediately or stored long- term in undiluted working aliquots at -20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze/thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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