Omentin, the product of a a recently recognized gene, is localized in the mental tissue (visceral adipose tissue). Omentin is present in the stromal vascular cells in the adipose tissue rather than in the adipocytes. It is predominantly expressed in the visceral adipose tissue than the subcutaneous tissue, with the omentin mRNA being 150 times higher in the visceral adipose tissue. Omentin has also been detected in human blood using Western blot analysis, and seems to increase insulin-stimulated glucose uptake in 3T3-L1 adipocytes in mice. It seems to increase Akt phosphorylation irrespective of insulin presence. Its role in glucose metabolism and obesity remains to be described; an insulin-sensitizing action is possible. Differences in Omentin expression has been noted in adipose tissue from normal patients and patients with inflammatory bowel disease although its significance is unknown. Human recombinant Omentin produced in E. coli is a single, polypeptide chain of 313 amino acids having a molecular mass of 35 kDa. It has been purified by using proprietary chromatographic techniques.
Human ITLN1 expressed in E.coli
CAT# CSC-CTK0420-10 (10 μg); CAT# CSC-CTK0420-50 (50 μg)
Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.
Lyophilized from a protein solution in 5 mM sodium phosphate buffer (pH 7.5) and 0.5% manntiol.
Please centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be stored long-term in undiluted working aliquots at –20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
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