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ActoFactor™ Recombinant Human Insulin Like Growth Factor 2

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Cat.No.
CSC-CTK0210
Description
IGF is a well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like and mitogenic activities. This growth factor has a major, but not absolute, dependence on Somatotropin. It is believed to be mainly active in adults in contrast to Insulin like Growth Factor 2, which is a major fetal growth factor. Recombinant human IGF-II produced in E. coli is a single, non-glycosylated polypeptide chain of 67 amino acids and a molecular mass of 7,505 Dalton.
Species
Human
Product Overview
Human IGF2 expressed in E.coli
Molecular Mass
7.5 kDa
Size
CAT# CSC-CTK0210-50 (50 μg); CAT# CSC-CTK0210-250 (250 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
Activity
Recombinant human Insulin Like Growth Factor-II is fully biologically active when compared to standards. The ED50, calculated by the competitive binding of IGF-II plasma derived to human placental membrane, is less then 1.0 ng/ml, corresponding to a specific activity of 10^6 IU/mg.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Sterile-filtered, white, lyophilized (freeze-dried) powder. The protein was lyophilized with no additives.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IGF-II should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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