Recombinant human Insulin produced in E. coli is a two chain, non-glycosylated polypeptide chain of 51 amino acids and a molecular mass of 5,807 Dalton.
Human INS expressed in E.coli
CAT# CSC-CTK0132-25 (25 mg); CAT# CSC-CTK0132-125 (125 mg)
Greater than 98% as determined by SDS-PAGE and RP-HPLC analysis.
Recombinant Insulin is fully biologically active when compared to a World Health Organization (WHO) reference standard which activity is 28 units/mg.
Less than 1 EU/μg.
Lyophilized (freeze-dried) powder. Recombinant human Insulin was lyophilized from a concentrated (1 mg/ml) solution with no additives.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu Insulin should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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