GHBP is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. A common alternate allele of this gene, called GHRd3, lacks exon three and has been well-characterized. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. Other splice variants, including one encoding a soluble form of the protein (GHRtr), have been observed but have not been thoroughly characterized. Recombinant human GHBP produced in E. coli is a single, non-glycosylated polypeptide chain of 237 amino acids and a molecular mass of 28,107 Dalton. It has been purified by proprietary chromatographic techniques.
Human IGFBP3 expressed in E.coli
CAT# CSC-CTK0554-20 (20 μg); CAT# CSC-CTK0554-100 (100 μg)
Greater than 98% as determined by SDS-PAGE and SEC-HPLC analysis.
GHBP is fully biologically active as evidenced by its ability of forming a 2:1 complex with HGH.
Less than 1 EU/μg.
Sterile-filtered, white, lyophilized (freeze-dried) powder. The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.0045 mM NaHCO3.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu GHBP should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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