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ActoFactor™ Recombinant Human Growth & Differentiation Factor 7

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Cat.No.
CSC-CTK0541
Description
GDF-7 belongs to the TGF-β superfamily of growth and differentiation factors. It is expressed selectively by roof plate cells that are located in the developing embryonic central nervous system, and has been shown to influence the neuronal identity of cells within the central nervous system. GDF-7 has also been implicated in the formation, maintenance, and repair of certain cartilage and ligament tissue. Recombinant human GDF-7 is a 28 kDa disulfide linked homodimer containing two 129 amino acid residues.
Species
Human
Product Overview
Human GDF7 expressed in E.coli
Molecular Mass
28 kDa
Size
CAT# CSC-CTK0541-10 (10 μg); CAT# CSC-CTK0541-50 (50 μg)
Expression System
E.coli
Purity
Greater than 98% as determined by HPLC and SDS-PAGE analysis.
Activity
Determined by its ability to inhibit induced alkaline phosphatase production by ATDC-5 chondrogenic cells.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Sterile filtered through a 0.2 micron filter and lyophilized from 10 mM citric acid.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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