USA USA: 1-631-626-9181 Europe Europe: 44-207-097-1828  
Search

Tissue samples, tissue arrays, cells, microorganisms, probes and services for your research.

Web Search    Google
Featured Products
Ordering Information

Payment methods we support:
✭ Invoice / Purchase Order
✭ Credit card

ActoFactor™ Recombinant Human Granulin

  • Specification
  • Recommended Products
Cat.No.
CSC-CTK0121
Description
The 88-kDa Progranulin, also called proepithelin is a PC cell-derived growth factor. It is a single precursor protein of granulins which are a family of secreted, glycosylated peptides. They are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. Granulins are a variety of active, 6 kDa peptides named granulin A (epithelin 1), granulin B (epithelin 2), granulin C, etc. Both the peptides and intact progranulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.|Human recombinant Progranulin fused to FLAG at its C-terminus and produced in HEK cells is a single, glycosylated polypeptide chain of 593 amino acids having a molecular mass of 88 kDa. It is purified by standard chromatographic techniques.
Species
Human
Product Overview
Human GRN expressed in HEK cells
Molecular Mass
88 kDa
Size
CAT# CSC-CTK0121-10 (10 μg); CAT# CSC-CTK0121-50 (50 μg)
Expression System
HEK cells
Purity
Greater than 90% as determined by SDS-PAGE and RP-HPLC analysis.
Activity
1. The effects of phospho-ERK1/2 and non-phospho-ERK1/2 on human Progranulin-FLAG in THP-1 cells were measured. To examine the signal of phospho-p44/42 MAPK and p44/42 MAP kinase, reactions were carried out at 37°C over 0, 30 and 60 minutes, respectively, by adding the recombinant protein (100 ng/ml) to the THP-1 monocyte cell, which were maintained with serum starvation for 24 hours. Recombinant proteins in lanes 1, 2, and 3 were subjected to THP- 1 monocyte cell treatments over 0, 30 and 60 minutes, respectively. 2. The effects of phospho- ERK1/2 and non-phospho-ERK1/2 on human Progranulin-FLAG in MCF10A cells were neasured. To examine the signal of phospho-p44/42 MAPK and p44/42 MAP kinase, reactions were carried out at 37°C over 0, 10, 30 and 60mins, respectively, by adding the recombinant protein (500 ng/ml) to MCF10A human breast epithelial cells, which were maintained with serum starvation for 24 hours. Recombinant proteins in lanes 2, 3 and 4 were subjected to MCF10A human breast epithelial cell treatments over 0, 10, 30 and 60 minutes, respectively.
Endotoxin Level
N/A
Formulation
Sterile-filtered clear solution containing 1XPBS.
Reconstitution
Please centrifuge product briefly before opening vial. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored dessicated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at –20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
Customer Support & Price Inquiry
Please input "bioarray" as verification code.
CONTACT US USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: 

Europe
Tel: 44-207-097-1828
Email: