Ghrelin/Obestatin is a hormone that is produced in the cells lining the stomach and small intestine of several mammals including humans; it drastically reduces appetite in mice and is expected to do the same in humans. Obestatin is a peptide hormone - a relatively small protein. It is encoded by the same gene that also encodes ghrelin, a peptide hormone that increases appetite. The protein produced by that gene breaks into two smaller peptides, ghrelin and obestatin. Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Ghrelin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 and is involved in satiety and decreased food intake. Human recombinant Ghrelin contains 115 amino acids (24-117 a.a.) and has a total molecular mass of 12.8 kDa. It is fused to a 20 amino acid His-tag at the N-terminus and purified by proprietary chromatographic techniques.
Human GHRL expressed in E.coli
CAT# CSC-CTK0646-25 (25 μg); CAT# CSC-CTK0646-125 (125 μg)
Greater than 90% as determined by SDS-PAGE analysis.
Less than 1 EU/μg.
Sterile-filtered, colorless solution. The Ghrelin p rotein solution contains 20 mM Tris-HCl (pH-8), 10% glycerol.
Please centrifuge product briefly before opening vial. The dissolved protein can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
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