Fibroblast Growth Factors (FGFs) comprise a family of at least eighteen structurally realted proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentation, angiogenesis, wound healing and tumorgenesis. The biological activities of the FGFs are mediated by a family if type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding. Four distinct genes encoding closely related FGF receptors, FGFR-1 to -4 are known. Multiple forms of FGFR-1 to -3 are generated by alternative splicing of the mRNAs. Recombinant human soluble FGFR-2a (IIIc) was fused via a Xa cleavage site with the Fc part of human IgG1. Recombinant human soluble FGFR-4 was fused with the Fc part of human IgG1. Human recombinant soluble FGFR-4/Fc is a disulfide-linked heterodimeric protein. The reduced form of human FGFR-4/Fc is a monomer with a calculated molecular mass of approximately 65 kDa. As a result of glycosylation, the recombinant protein has a mass of 80-85 kDa.
Human FGFR4 expressed in E.coli
CAT# CSC-CTK0166-10 (10 μg); CAT# CSC-CTK0166-50 (50 μg)
Greater than 90% as determined by SDS-PAGE analysis.
Determined by its ability to inhibit human FGF acidic-dependent proliferation on HUVE cells. The ED50 for this effect is typically at 15-30 ng/ml.
Less than 1 EU/μg.
Lyophilized from a sterile-filtered solution with no additives.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water or PBS to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu sFGFR-4 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
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