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ActoFactor™ Recombinant Human Fibroblast growth factor 5

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Cat.No.
CSC-CTK0143
Description
FGF-5 is a secreted heparin binding growth factor that belongs to the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-5 binds to FGFR 1c and 2c, and plays a regulatory role in the hair growth cycle. Recombinant human FGF-5 is a 27.6 kDa protein consisting of 252 amino acid residues.
Species
Human
Product Overview
Human FGF5 expressed in E.coli
Molecular Mass
27.6 kDa
Size
CAT# CSC-CTK0143-50 (50 μg); CAT# CSC-CTK0143-250 (250 μg)
Expression System
E.coli
Purity
Greater than 95% as determined by SDS-PAGE analysis.
Activity
The ED50, as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors, is 2 x 10^6 units/mg.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Sterile, lyophilized (freeze-dried) powder. Lyophilzed from a solution in 2X PBS buffer (pH 7.2).
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized FGF-5 should be reconstituted in sterile 1X PBS (pH 7.2) to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into water or other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu FGF-5 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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