FGF-4 is a heparin binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-4 signals through the FGFR 1c, 2c, 3c, and 4. Recombinant human FGF-4 is a 19.7 kDa protein consisting of 182 amino acid residues.
Human FGF4 expressed in E.coli
CAT# CSC-CTK0141-25 (25 μg); CAT# CSC-CTK0141-125 (125 μg)
Greater than 95% as determined by SDS-PAGE analysis.
The ED50, as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors, is 2 x 10^6 units/mg.
Less than 1 EU/μg.
Sterile, lyophilized (freeze-dried) powder. Lyophilzed from a solution in 10 mM Tris, pH 7.4 + 100 mM NaCl.
Please centrifuge product briefly before opening vial. The lyophilized FGF-4 should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu FGF-4 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
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