Recombinant Human Erythropoietin-alpha, produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology, is a single polypeptide chain of 165 amino acids, a predicted molecular mass of 21,000 Dalton and an apparent glycosylated molecular mass of 36-40 kDa.
Human EPO expressed in CHO cells
CAT# CSC-CTK0098-50 (50 μg); CAT# CSC-CTK0098-250 (250 μg)
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
Erythropoietin-alpha is fully biologically active when compared to a standard. The specific activity was measured by Normocyth-aemic mice and was found to be 150,000 IU/mg.
Less than 1 EU/μg.
Sterile-filtered, white, lyophilized (freeze-dried)powder. Recombinant Erythropoietin-alpha was lyophilized after extensive dialysis against a buffer containing 0.59 mg sodium citrate, 0.58 mg sodium chloride and 0.006 mg citric acid per liter.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu EPO-alpha should be used immediately or stored long- term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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