EBI-3 has an induced expression in B lymphocytes in reaction to Epstein-Barr virus infection. EBI- 3 encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form IL-27. EBI-3 drives rapid clonal expansion of naive cd4(+) T-cells. EBI-3 strongly synergizes with IL-12 to activate IFN-gamma production of naive cd4(+) T-cells. EBI-3 mediates its biologic effects through the cytokine receptor WSX-1/TCCR. Human recombinant EBI-3 produced in E. coli is a single, non-glycosylated, polypeptide chain containing 209 amino acids fragment (21-229) having a molecular weight of 34 kDa and fused with a 4.5 kDa amino-terminal hexahistidine tag.
Human EBI3 expressed in E.coli
CAT# CSC-CTK0603-10 (10 μg); CAT# CSC-CTK0603-50 (50 μg)
Greater than 95% as determined by SDS-PAGE analysis.
Sterile-filtered, clear protein solution in 1X PBS, 50% glycerol.
Please centrifuge product briefly before opening vial. The dissolved protein can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
Store at 4°C if entire vial will be used within 4 weeks. Store frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.