Recombinant Human Epidermal Growth Factor (rHu EGF) is a polypeptide growth factor, which stimulates the proliferation of a wide range of epidermal and epithelial cells. rHu EGF produced in E. coli is a single, non-glycosylated polypeptide chain of 53 amino acids and a molecular mass of 6,222 Dalton.
Human EGF expressed in E.coli
CAT# CSC-CTK0126-500 (500 μg); CAT# CSC-CTK0126-2500 (2.5 mg)
Greater than 98% as determined by SDS-PAGE and SEC-HPLC analysis.
Greater than 1.0×10^7 IU/mg
Less than 1 EU/μg.
Lyophilized from a sterile-filtered, concentrated protein solution in PBS (pH 7.4) with no additives.
Please centrifuge product briefly before opening vial. The lyophilized rHu EGF should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, should be stored desiccated at -20°C. Reconstituted rHu EGF should be used immediately or stored in undiluted working aliquots at -20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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