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ActoFactor™ Recombinant Human Cytotoxic T-lymphocyte-associated protein 4

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Cat.No.
CSC-CTK0315
Description
CTLA-4 (CD152) is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have also been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. CTLA-4 is expressed in low copy number by T-cells only after activation, but it binds the CD28 ligand with approximately 20-fold higher affinity than CD28. A soluble form of the extracellular domain of CTLA-4 has been shown to bind the CD28 ligand with high affinity and to suppress T-cell-dependent antibody responses in vivo. Large doses of this soluble protein also suppress responses to a second immunization. |Human recombinant CTLA-4 produced in insect cells is a homodimeric, glycosylated polypeptide chain( having a total molecular mass of 80 kDa. Each subunit (40 kDa) is fused via a polypeptide linker to the Fc portion of human IgG1. It has been purified by using proprietary chromatographic techniques.
Species
Human
Product Overview
Human CTLA4 expressed in Sf9 insect cells
Molecular Mass
2 x 40 kDa
Size
CAT# CSC-CTK0315-25 (25 μg); CAT# CSC-CTK0315-125 (125 μg)
Expression System
Sf9 insect cells
Purity
Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.
Activity
N/A
Endotoxin Level
N/A
Formulation
Lyophilized from a sterile-filtered protein solution with no additives.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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