Recombinant Human Granulocyte and Macrophage Colony Stimulating Factor (rHu GM-CSF) is a potent species specific stimulator of bone marrow cells. It is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. GM-CSF also stimulates hematopoietic precursor cells of granulocytes, macrophages, eosinophils and erythrocytes. Human GM-CSF produced in yeast is a single, glycosylated, polypeptide chain of 127 amino acids and a molecular mass of 26-32 kDa. rhu GM-CSF differs from the natural human GM-CSF by a substitution of leucine at position 23 (R to L), and the carbohydrate moiety may be different from the native protein. Recombinant GM-CSF has been purified by proprietary chromatographic techniques.
Human CSF3 expressed in Pichia pastoris
CAT# CSC-CTK0153-10 (10 μg); CAT# CSC-CTK0153-50 (50 μg)
Greater than 97% as determined by SDS-PAGE and HPLC analysis.
rHu GM-CSF is fully biologically active when compared to a standard. The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is 5 x 10^6 units/mg.
Less than 1 EU/μg.
The protein was lyophilized from a concentrated (1 mg/ml) solution containing 10 mM phosphate buffer (pH 7.0), 40 mg mannitol and 10 mgsucrose.
Please centrifuge product before opening the vial. The lyophilized protein should be reconstituted in water to a concentration of 0.1-1.0 mg/ml. For further dilution carrier protein should be added to avoid loss of bioactivity.
Storage & Stability
The lyophilized powder, though stable at room temperature, is best stored desiccated at -20°C. Reconstituted rHu GM-CSF should be stored in working aliquots at -20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze/thaw cycles.
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