Recombinant Human Granulocyte Stimulating Factor (rHu G-CSF) is a potent stimulator of bone marrow cells, especially those of neutrophil lineage. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF. In epithelial, endothelial, and fibroblastic cells secretion of G-CSF is induced by Interleukin-17. Human G-CSF is an 20 kDa glycosylated protein containing 174 amino acid residues.
Human CSF3 expressed in CHO cells
CAT# CSC-CTK0160-10 (10 μg); CAT# CSC-CTK0160-50 (50 μg)
Greater than 97% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
Recombinant G-CSF is fully biologically active when compared to a standard. The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake), is less than 0.07 ng/ml, corresponding to a specific activity of 1.27 x 10^8 IU/mg.
Less than 1 EU/μg.
Human G-CSF was lyophilized from a concentrated (1 mg/ml) Phosphate-Buffered Saline, pH 7.4.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu G-CSF should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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