USA USA: 1-631-626-9181 Europe Europe: 44-207-097-1828  
Search

Tissue samples, tissue arrays, cells, microorganisms, probes and services for your research.

Web Search    Google
Featured Products
Ordering Information

Payment methods we support:
✭ Invoice / Purchase Order
✭ Credit card

ActoFactor™ Recombinant Human Colony stimulating factor 2 (granulocyte-macrophage)

  • Specification
  • Recommended Products
Cat.No.
CSC-CTK0076
Description
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a potent species-specific stimulator of granulocyte-macrophage, eosinophil, megakaryocyte and erythroid progenitors. Human GM-CSF is a 14 kDa protein containing 127 amino acid residues.
Species
Human
Product Overview
Human CSF2 expressed in E.coli
Sequence
A DNA sequence encoding the mature human GM-CSF protein (Ala 18 - Glu 144; Accession #CAA26822) was expressed in E. coli.
Size
CAT# CSC-CTK0076-10 (10 μg); CAT# CSC-CTK0076-50 (50 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity
The biological activity of recombinant human GM-CSF is measured in a cell proliferation assay using a factor-dependent cell line, TF-1. The ED50 for this effect is typically 6 - 30 pg/mL.
Endotoxin Level
Endotoxin level is <1.0 EU per 1 μg cytokine, as determined by the LAL method.
Formulation
Recombinant human GM-CSF is lyophilized from a 0.2 μm filtered solution in phosphate buffered saline (PBS) containing 50 μg bovine serum albumin per 1 μg cytokine.
Reconstitution
Reconstitute human GM-CSF at a concentration greater than 10 μg/mL with sterile PBS containing at least 0.1% human or bovine serum albumin.
Storage & Stability
Lyophilized human GM-CSF is stable for up totwelve months from date of receipt at -20°C to -70°C. Reconstituted human GM-CSF can be stored under sterile conditions at 2°C - 8°C for one month, or at -20°C to -70°C (in a manual defrost freezer) for three months without detectable loss of activity. Avoid repeated freezing and thawing.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
Customer Support & Price Inquiry
Please input "bioarray" as verification code.
CONTACT US USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: 

Europe
Tel: 44-207-097-1828
Email: