Macrophage-Colony stimulating factor (M-CSF or CSF-1) can be produced by a number of cells including fibroblasts, activated macrophages, secretory epithelial cells of the endometrium, bone marrow stromal cells, and LPS- or cytokine-activated endothelial cells. M-CSF not only stimulates the formation of macrophage colonies from bone marrow hematopoietic progenitor cells, but also induces proliferation of isolated macrophages, enhances macrophage antibody-dependent cell-mediated cytotoxicity, induces priming and enhancing of macrophages ability to kill tumor cells and microorganisms, regulates release of cytokines and other inflammatory modulators from macrophages, stimulates pinocytosis and osteoclast differentiation. M-CSF is synthesized as a membrane-bound propeptide. Subsequent to proteolytic processing, M-CSF can occur either as a secreted, soluble form or as a membrane-anchored form, both of which are biologically active. Natural M-CSFs are glycosylated, disulfide-linked, homodimeric proteins with molecular weights ranging from 40-70 kDa. Several C-terminal variants of the soluble form of M-CSF have been found. The N-terminal 150 amino acid residues of the mature M-CSF, a region necessary and sufficient for interaction with the M-CSF receptor, is highly conserved (80% homology) between the human and murine proteins. Human M-CSF is active in the murine system, but murine M-CSF is species-specific. M-CSF is a glycoprotein, but the carbohydrate moiety is not necessary for biological activity.
Human CSF1 expressed in E.coli
A DNA sequence encoding the N-terminus 158 amino acid residues of the extracellular domain of the mature native human M-CSF protein sequence was expressed in E. coli.
CAT# CSC-CTK0026-5 (5 μg); CAT# CSC-CTK0026-25 (25 μg)
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity was determined by cell proliferation assay in M-NFS-60 cells and the ED50 using this assay was typically 0.5 – 1.5 ng/ml.
Endotoxin level is less than 0.1 ng per mg of the cytokine, as determined by the LAL method.
Lyophilized from a sterile-filtered solution of phosphate-buffered saline containing 50 mg of bovine serum albumin per 1 mg of cytokine.
It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 1 mg/ml of the cytokine.
Storage & Stability
The lyophilized sampleis stable for greater than six months at -20 °C to -70°C. Reconstituted M-CSF can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to-70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.