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ActoFactor™ Recombinant Human Chemokine (C-X-C motif) ligand 1

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Cat.No.
CSC-CTK0001
Description
GROa is a member of the a (CXC) family of chemokines. It is produced by monocytes, neutrophils, mast cells, epithelial cells, endothelial cells, keratinocytes and tumor cells. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF. GROa is a chemoattractant for neutrophils, lymphocytes, monocytes, and epidermal melanocytes. In addition, GROa induces activation of neutrophils and basophils, has angiogenic effects, inhibits erythrocyte invasion and decreases the expression of interstitial collagen. GROa binds to IL-8R-A, IL-8R-B and DARC. Human GROa cDNA encodes a 107 amino acid residue precursor protein containing 34 N-terminal amino acid residues that are cleaved to generate the mature protein. The recombinant maturehuman GROa contains 73 amino acid residues and has an apparent molecular mass of 7.9 kDa.
Species
Human
Product Overview
Human CXCL1 expressed in E.coli
Sequence
A DNA sequence encoding the mature human GROa protein was expressed in E. coli.
Size
CAT# CSC-CTK0001-10 (10 μg); CAT# CSC-CTK0001-50 (50 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity
Activity was determined by ability to induce myeloperoxidase release from cytochalasin treated neutrophils, or chemotaxis of mouse BaF/3 cells transfected with human CXCR2. The ED50 for these effects were typically 0.15 - 0.3 mg/mL or 1 - 4 ng/mL, respectively.
Endotoxin Level
Endotoxin level less than 0.1 ng per 1 mg of the cytokine as determined by the LAL method.
Formulation
Lyophilized from a sterile solution in 10% acetonitrile, 0.1% TFA containing 50 mg of human serum albumin per 1 mg of cytokine.
Reconstitution
It is recommended that sterile 4 mM HCl containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 mg/mL of the cytokine.
Storage & Stability
Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted human CXCL1 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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