Recombinant Human RANTES (rHu RANTES) is a CC-chemokine that can signal through the CCR1, CCR3, CCR5 and US28 (cytomegalovirus receptor) receptors. It is a chemoattractant towards monocytes, memory T cells (CD4+/CD45RO), basophils, and eosinophils. RANTES also has the capability to inhibit certain strains of HIV-1, HIV-2 and simian immunodeficiency virus (SIV). Recombinant human RANTES is a single non-glycosylated 7.81 kDa polypeptide chain containing 68 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.
Human CCL5 expressed in E.coli
CAT# CSC-CTK0377-20 (20 μg); CAT# CSC-CTK0377-100 (100 μg)
Greater than 98% as determined by SDS-PAGE and RP-HPLC analysis.
The activity was determined by chemoatractting human blood monocytes at a concentration between 1-10 ng/ml.
Less than 1 EU/μg.
Lyophilized from a concentrated (1 mg/ml), sterile-filtered protein solution with no additives.
Please centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored dessicated at -20°C. Reconstituted rHu RANTES should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
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