LAG-1 is CC chemokine that signals through the CCR5 receptor. LAG-1 is identical to MIP-1beta (ACT II isotype) except for two amino acid substitutions; arginine for histidine at position 22 and serine for glycine at position 47 of the mature protein. LAG-1 chemoattracts monocytes, and exhibits activity as an HIV suppressive factor. Recombinant human LAG-1 is a 7.7 kDa protein containing 69 amino acid residues.
Human CCL4L1 expressed in E.coli
CAT# CSC-CTK0461-20 (20 μg); CAT# CSC-CTK0461-100 (100 μg)
Greater than 98% as determined by SDS-PAGE analysis.
Determined by its ability to chemoattract CCR5 transfected cells using a concentration of 0.1- 10.0 ng/ml.
Less than 1 EU/μg.
Lyophilized (freeze-dried), sterile powder. Lyophilized with no additives.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1 - 1,0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu LAG-1 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
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