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ActoFactor™ Recombinant Human Chemokine (C-C motif) ligand 22 (67 aa variant)

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Cat.No.
CSC-CTK0575
Description
MDC is a CC chemokine that is produced in B cells, macrophages, monocyte-derived dendritic cells, activated NK cells and CD4 T cells. It signals through the CCR4 receptor. MDC chemoattracts monocytes, dendritic cells and NK cells and exerts HIV suppressive activity. The 67 amino acid form of MDC displays reduced chemoattractant activity but retains HIV suppressive activity. Recombinant human MDC is an 8.0 kDa protein containing 67 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.
Species
Human
Product Overview
Human CCL22 expressed in E.coli
Molecular Mass
8.0 kDa
Size
CAT# CSC-CTK0575-20 (20 μg); CAT# CSC-CTK0575-100 (100 μg)
Expression System
E.coli
Purity
Greater than 99% as determined by SDS-PAGE and HPLC analysis.
Activity
Determined by its ability to chemoattract human T cells using a concentration range of 10.0- 100.0 ng/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized from a sterile-filtered solution with no additives.
Reconstitution
Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted MDC should be used immediately or stored in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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