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ActoFactor™ Recombinant Human Chemokine (C-C motif) ligand 2
- Specification
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Cat.No.
CSC-CTK0038
Description
Monocyte chemotactic protein-1 (MCP-1) is a member of the b (CC) family of chemokines. The protein is identical to the product of the human JE gene which is a platelet-derived growth factor (PDGF)-inducible gene. MCP-1 is produced by fibroblasts, tumor cells, smooth muscle cells, endothelial cells, and mononuclear phagocytes. MCP-1 is a chemoattractant for monocytes. In addition, it activates monocytes to be cytostatic for some human tumor cells lines; it increases cytosolic free calcium; it generates and releases monocyte superoxide anions and monocyte lysosomal enzymes in vitro. MCP-1 was reported to be capable of regulating adhesion molecule expression and cytokine production in human monocytes as well as chemoattracting, activating, and inducing histamine release from basophils. MCP-1 can bind to CCR1 and a specific receptor cloned from THP-1 and MonoMac 6 cells. Human MCP-1 cDNA encodes a 99 amino acid residue precursor protein with a 23 residue hydrophobic signal peptide that is cleaved to generate the 76 residue mature protein. The recombinant mature protein has a predicted molecular mass of approximately 8.7 kDa.
Species
Human
Product Overview
Human CCL2 expressed in E.coli
Sequence
A DNA sequence encoding mature human MCP-1 was expressed in E. coli.
Size
CAT# CSC-CTK0038-10 (10 μg); CAT# CSC-CTK0038-50 (50 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity
Activity was determined by monocyte chemotaxis and the ability to chemoattract mouse BaF/3 cells transfected with human CCR2A. The ED50 for these effects were typically 5 - 20 ng/mL and 10 - 60 ng/mL, respectively.
Endotoxin Level
Endotoxin level less than 0.1 ng per 1 mg of the cytokine as determined by the LAL method.
Formulation
Lyophilized from a sterile solution in PBS containing 50 mg of bovine serum albumin per 1 mg of cytokine.
Reconstitution
It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 1 mg/mL of the cytokine.
Storage & Stability
Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted human MCP-1/CCL2 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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