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ActoFactor™ Recombinant Human Chemokine (C-C motif) ligand 15

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Cat.No.
CSC-CTK0466
Description
MIP-5 is a CC chemokine that is expressed in the heart, skeletal muscle and adrenal gland. MIP-5 primarily signals through he CCR1 receptor, but also has been found to bind to CCR3. MIP-5 is chemotactic towards T cells and monocytes. Recombinant human MIP-5 is a 10.1 kDa protein containing 92 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.
Species
Human
Product Overview
Human CCL15 expressed in E.coli
Molecular Mass
10.1 kDa
Size
CAT# CSC-CTK0466-25 (25 μg); CAT# CSC-CTK0466-125 (125 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE and HPLC analysis.
Activity
Determined by its ability to chemoattract human T lymphocytes using a concentration range of 1.0-10.0 ng/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized from a sterile-filtered concentrated protein solution (1 mg/ml) containing 20 mM PBS (pH 7.4) and 100 mM NaCl.
Reconstitution
Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted MIP-5 should be used immediately or stored in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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