MIP-5 is a CC chemokine that is expressed in the heart, skeletal muscle and adrenal gland. MIP-5 primarily signals through he CCR1 receptor, but also has been found to bind to CCR3. MIP-5 is chemotactic towards T cells and monocytes. Recombinant human MIP-5 is a 10.1 kDa protein containing 92 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.
Human CCL15 expressed in E.coli
CAT# CSC-CTK0466-25 (25 μg); CAT# CSC-CTK0466-125 (125 μg)
Greater than 97% as determined by SDS-PAGE and HPLC analysis.
Determined by its ability to chemoattract human T lymphocytes using a concentration range of 1.0-10.0 ng/ml.
Less than 1 EU/μg.
Lyophilized from a sterile-filtered concentrated protein solution (1 mg/ml) containing 20 mM PBS (pH 7.4) and 100 mM NaCl.
Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted MIP-5 should be used immediately or stored in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.
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