Recombinant Human Cardiotrophin-1 (rHu CT-1) is a member of the IL-6 family of cytokines which also includes LIF, CNTF, OSM (Oncostatin M), IL-11, IL-6 and possibly NT-1/ BSF-3. CT-1 is a pleiotropic cytokine which is expressed in various tissues including the adult heart, skeletal muscle, ovary, colon, prostate and fetal lung and signals through the LIF receptor and the gp130 receptor subunit. CT-1 has the ability to induce cardiac myocyte hypertrophy, and enhances the survival of cardiomyocyte and different neuronal populations. Biologically active human CT-1 is synthesized as a 201 amino acid polypeptide lacking a hydrophobic N-terminal secretion signal sequence. Recombinant Human Cardiotrophin-1 is a 21.5 kDa protein consisting of 200 amino acid residues.
Human CTF1 expressed in E.coli
CAT# CSC-CTK0557-10 (10 μg); CAT# CSC-CTK0557-50 (50 μg)
Greater than 98% as determined by SDS-PAGE and HPLC analyses.
The ED50 as determined by the dose-dependent proliferation of TF-1 cells was 1 x 10^6 units/mg.
Less than 1 EU/μg.
Lyophilized from a sterile-filtered (0.2 micron filter) protein solution in 1XPBS with no additives.
Please centrifuge product before opening the vial. The lyophilized protein should be reconstituted in sterile 20 mM Tris (pH 8.0) to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature, is best stored desiccated at -20°C. Reconstituted CT-1 should be stored in working aliquots at -20°C. Please avoid repeated freeze / thaw cycles. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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