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ActoFactor™ Recombinant Human CD4 molecule (125-202 aa variant)

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Cat.No.
CSC-CTK0122
Description
CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. Antibody to CD4 recognizes T-helper cells required for recognition of class II antigens. It reacts with ~60% of peripheral blood E rosette-positive (E+) cells while showing negligible reactivity with E-cells, monocytes, granulocytes, EBV-transformed B cell lines, and mouse splenocytes. Recombinant human CD4 produced in E. coli contains amino acids 125-202 and is fused with a 4 kDa His-Tag, having a total molecular weight of 19 kDa.
Species
Human
Product Overview
Human CD4 expressed in E.coli
Molecular Mass
19 kDa
Size
CAT# CSC-CTK0122-20 (20 μg); CAT# CSC-CTK0122-100 (100 μg)
Expression System
E.coli
Purity
Greater than 90% as determined by SDS-PAGE analysis.
Activity
N/A
Endotoxin Level
N/A
Formulation
Sterile-filtered, white, lyophilized (freeze-dried) powder. The protein was lyophilized from a protein solution in 1X PBS.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles. Can be used for protein assay, antibody production and Western blotting.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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