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ActoFactor™ Recombinant Human CD14 molecule (CHO)
- Specification
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Cat.No.
CSC-CTK0300
Description
CD14 (also known lipopolysaccharide [LPS] receptor) is expressed strongly on monocytes and macrophages and weakly on the surface of neutrophils. CD14 (1-201 a.a.) is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentrations as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation. The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wildtype CD14 is the 50 kDa protein.|The rHuCD14 is produced from human CD14-transfected CHO-cells. Before transfection, the complete human CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR.
Species
Human
Product Overview
Human CD14 expressed in CHO cells
Molecular Mass
50 kDa
Size
CAT# CSC-CTK0300-50 (50 μg); CAT# CSC-CTK0300-250 (250 μg)
Expression System
CHO cells
Purity
Greater than 95% as determined by SDS-PAGE analysis.
Activity
Up to 20 μg/ml CD14 inhibit binding of FITC-LPS (0.5 μg/ml) to 6 x 10^5 CD14+CHO transfectants (FACS).
Endotoxin Level
N/A
Formulation
Recombinant human CD-14 was lyophilized from a concentrated protein solution (1 mg/ml) containing phosphate-buffered saline, pH 7.2.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1 - 1,0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored dessicated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at –20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles... Up to 20 μg/ml CD14 inhibit binding of FITC-LPS (0.5 μg/ml) to 6 x 10? CD14+CHO transfectants (FACS).
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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