Recombinant Human Brain-Derived Neurotrophic Factor (rHu BDNF) is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. The active form of human BDNF is a 27.0 kDa dimer, formed by two identical 119 amino acid subunits that is held together by strong hydrophobic interactions.
Human BDNF expressed in E.coli
CAT# CSC-CTK0483-10 (10 μg); CAT# CSC-CTK0483-50 (50 μg)
Greater than 97% as determined by SDS-PAGE and RP-HPLC analysis.
The ED50, as determined by the dose-dependent induction of ACHE (acetylcholine esterase) activity in rat basal forebrain primary septal cultures, was found to be 50 ng/ml corresponding to a specific activity of 20,000 units/mg.
Less than 1 EU/μg.
Lyophilized from a sterile-filtered protein solution with no additives.
Please centrifuge briefly before opening the vial. The lyophilized human BDNF should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into water or other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted human BDNF should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.
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