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ActoFactor™ Recombinant Human Betacellulin

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Cat.No.
CSC-CTK0175
Description
BTC is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. The effects of betacellulin are probably mediated by the egf receptor and other related receptors.|Human recombinant Betacellulin produced in E. coli is a single, non-glycosylated polypeptide chain of 80 amino acids having a molecular mass of 9 kDa. It is purified by proprietary chromatographic techniques.
Species
Human
Product Overview
Human BTC expressed in E.coli
Molecular Mass
19.7 kDa
Size
CAT# CSC-CTK0175-20 (20 μg); CAT# CSC-CTK0175-100 (100 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE and HPLC analysis.
Activity
The ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells (measured by 3H-thymidine uptake), is 2.0×10^7 IU/mg.
Endotoxin Level
N/A
Formulation
Lyophilized (freeze-dried) powder. Sterile-filtered Betacellulin was lyophilized after extensive dialysis against 20 mM phosphate buffer (pH 7.4).
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into water or other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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