Established in vitro from the AS-30-D tumor ascites (by CLS Cell Lines Service), RAP-Test negative. The species was confirmed by Real-Time PCR.
Hypodiploid rat karyotype with 12% tetraploidy; 38 (35-41).
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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