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AC-1M32
- Specification
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Cat.No.
CSC-C0475
Description
One of five phenotypically and cytogenetically distinct subclones of a hybrid (ACH1P) formed by fusing an HGPRT-defective mutant (AC1-1) of JEG-3 human choriocarcinoma with primary human trophoblast (chorion laeve) cells
Species
Human
Source
choriocarcinoma-trophoblast hybrid
Recommended Medium
Ham's F-12 and 10% h.i. FBS.
Culture Properties
monolayer
Morphology
epitheloid cells growing adherently in monolayers
Karyotype
Human flat-moded hypertetraploid karyotype with 4% polyploidy - 80-104<4n>XXXY, +1, +2, +3, -4, +7, +9, -13, -13, -15, +16, +16, -18, -20, +21, +22, +5-7mar, del(X)(p11p22), der(X)(Xpter->Xq2?8::4p1?->4p1?::11q1?1->11qter), del(1)(p11), der(7)t(7;7)(p22;q
Quality Control
Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, desmin -, endothel -, GFAP +, neurofilament -, vimentin -
Viruses: PCR: EBV -, HBV -,
Immunology: cytokeratin +, desmin -, endothel -, GFAP +, neurofilament -, vimentin -
Viruses: PCR: EBV -, HBV -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
What is the role of EMEM in cell culture?
A:
EMEM provides a balanced nutritional environment that supports the growth, metabolism, and viability of a wide range of mammalian cells in vitro.
Average Rating: 4.0 | 1 Scientist has reviewed this product
An excellent choice
The compatibility and reliability of these products have significantly enhanced our cellular applications, supporting the execution of complex experimental techniques with precision and confidence.
08 Feb 2023
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