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A375;A-375

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Cat.No.
CSC-C8234L
Description
The A375 cell line was established by D.J. Giard in 1973. This cell line was derived from a 54 year old female with malignant melanoma. The cell line produces rapidly growing amelanotic melanomas in anti-thymocyte serum treated NIH Swiss mice. According to our sequencing, WB and PCR results this cell line carries a BRAF V600E mutation.
Tissue of Origin
Skin
Organism
Homo sapiens (human)
STR DNA Profile
Amelogenin: X,X
CSF1PO: 11,12
D13S317: 11,14
D16S539: 9
D5S818: 12
D7S820: 9
THO1: 8
TPOX: 8,10
vWA: 16,17
D3S1358: 15,17
D21S11: 29,30
D18S51: 12,17
Penta E: 10,12
Penta D: 9,15
D8S1179: 11,14
FGA: 23
Karyotype
It is a hypotriploid with a modal number of 62 chromosomes. 9 marker chromosomes that could be commonly found in each cell, and normal N2, N6, and N22 are present at one copy per cell.
Culture Properties
Adherent
Morphology
epithelial
Recommended Medium
DMEM and 10% h.i.FBS.
Quality Control
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
liquid nitrogen vapor phase
Application
The A375 cell line can be used for tumourigenicity studies, it also can be used as a suitable transfection host.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CM-1010X
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