The A375 cell line was established by D.J. Giard in 1973. This cell line was derived from a 54 year old female with malignant melanoma. The cell line produces rapidly growing amelanotic melanomas in anti-thymocyte serum treated NIH Swiss mice. According to our sequencing, WB and PCR results this cell line carries a BRAF V600E mutation.
Homo sapiens (human)
STR DNA Profile
Penta E: 10,12
Penta D: 9,15
It is a hypotriploid with a modal number of 62 chromosomes. 9 marker chromosomes that could be commonly found in each cell, and normal N2, N6, and N22 are present at one copy per cell.
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
liquid nitrogen vapor phase
The A375 cell line can be used for tumourigenicity studies, it also can be used as a suitable transfection host.
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