The A-427 cell line was established by D.J. Giard in 1973. The cells were established from the lung carcinoma of a 52-year-old Caucasian man.
Homo sapiens (human)
STR DNA Profile
D21S11 : 31.2
Penta E: 15,17
Penta D: 13
(P60) hypotriploid to hypertriploid with abnormalities including dicentrics, minutes and large subtelocentric marker.
Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Bacteria
specific PCR: negative
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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