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A-427

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Cat.No.
CSC-C0340
Description
The A-427 cell line was established by D.J. Giard in 1973. The cells were established from the lung carcinoma of a 52-year-old Caucasian man.
Tissue of Origin
Lung
Organism
Homo sapiens (human)
STR DNA Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 11,13
D5S818: 12
D7S820: 8,12
THO1: 9
TPOX: 8,11
vWA:17
D3S1358: 16
D21S11 : 31.2
D18S51:12
Penta E: 15,17
Penta D: 13
D8S1179: 12,13
FGA: 18
Karyotype
(P60) hypotriploid to hypertriploid with abnormalities including dicentrics, minutes and large subtelocentric marker.
Culture Properties
monolayer
Morphology
Epithelial
Recommended Medium
EMEM and 10% h.i. FBS
Quality Control
Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Bacteria
specific PCR: negative
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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