The A-204 cell line was established in 1973 by D.J. Giard. The cells were established from the rhabdomyosarcoma of a 1-year-old girl in 1973; reported to form small malignant tumors in nude mice consistent with embryonic rhabdomyosarcoma.
Homo sapiens (human)
STR DNA Profile
Penta E: 7,10
Penta D: 9,12
Diploidy and tetraploidy
Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Bacteria
specific PCR: negative
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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