The 3T6 cell line was established from 17 to 19 days old mouse embryos. The cells were established from disaggregated Swiss albino mouse embryos in 1963; cells were described to secrete collagen and hyaluronic acid. The species was confirmed by Real-Time PCR.
Mus musculus ,mouse
Murine flat-moded hypertriploid/hypotetraploid karyotype - 67(64-73)<3n>.
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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