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3T3-Swiss Albino; 3T3

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Cat.No.
CSC-C2039
Description
The 3T3 cell line was established from 17 to 19 days old mouse embryos. The cells are established from disaggregated Swiss albino mouse embryos in 1962; cells are used for transfection studies with DNA viruses and as assay system for transformation studies; cells have a high sensitivity to contact inhibition. A confluent monolayer yields 40.000 cells/cm2. The cells should be grown in plastic flasks; they do not grow well on some types of glass surfaces. A saturation density of approximately 50.000 cells/cm2 can be reached. The species was confirmed by Real-Time PCR.
Organism
Mus musculus ,mouse
Karyotype
Murine hypertriploid (near tetraploid sideline present) karyotype with 13% polyploidy - 68(58-73)<3n>.
Source
embryo
Culture Properties
monolayer
Morphology
fibroblast
Recommended Medium
DMEM and 10% h.i.FBS.
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6256W
CSC-6264W
CSC-6284W
CSC-6285W
CSC-6286W
CSC-6287W
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CONTACT US USA
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Tel: 1-631-626-9181
Fax: 1-631-614-7828
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Europe
Tel: 44-207-097-1828
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