The 3T3 cell line was established from 17 to 19 days old mouse embryos. The cells are established from disaggregated Swiss albino mouse embryos in 1962; cells are used for transfection studies with DNA viruses and as assay system for transformation studies; cells have a high sensitivity to contact inhibition. A confluent monolayer yields 40.000 cells/cm2. The cells should be grown in plastic flasks; they do not grow well on some types of glass surfaces. A saturation density of approximately 50.000 cells/cm2 can be reached. The species was confirmed by Real-Time PCR.
Mus musculus ,mouse
Murine hypertriploid (near tetraploid sideline present) karyotype with 13% polyploidy - 68(58-73)<3n>.
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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