Established from the primary tumor of a 72-year-old man with gastric adenocarcinoma in 1987
Human hypertetraploid karyotype with 12% polyploidy - 47(45-52)<2n>XY, +20, t(1;15)(p11;p11), t(6;12) (p21;q21), i(13q), t(13;14)(p10;q10) - closely resembles published karyotype
adherent epithelial cells growing as confluent monolayers
Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-1
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 0.7 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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EPAC1 overexpression is a prognostic marker and its inhibition shows promising therapeuticpotential for gastric cancer
Authors: Sun D P, Fang C L, Chen H K, et al.
The Oncology Reports, 2017,37(4): 1953-1960.
Overexpression of Mitochondrial GTPase MFN2 Represents a Negative Prognostic Marker in Human Gastric Cancer and Its Inhibition Exerts Anti-Cancer Effects
Authors: Fang C L, Sun D P, Chen H K, et al.
The Journal of Cancer, 2017, 8(7): 1153.