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VM-CUB1

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  • Cat.No.
  • CSC-C0446
  • Description
  • Established from the primary transitional cell carcinoma of the urinary bladder of a male patient; described to carry a p53 point mutation
  • Recommended Medium
  • 90% DMEM (4.5 g/L glucose) + 10% h.i. FBS
  • Organism
  • Human
  • Morphology
  • adherent epithelial-like cells growing as monolayers
  • Culture Properties
  • monolayer
  • Karyotype
  • Human hypertriploid karyotype with 4% polyploidy - 80<3n>XX/XX?Y, +1, +1, +5, +6, +7, +9, -11, +15, -18, +19, +20, +22, +22, +7mar, del(1)(q21), del(1)(q11), der(1;6)(p10;q10)x2, add(3)(p21.3)x2, i(5p), der(6)t(1;6)(q11;q22), ?inv(7)(p15q35), i(9q), del(1
  • Quality Control
  • Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
    Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokera
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • urinary bladder transitional cell carcinoma

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45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com