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TCC-SUP

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  • Cat.No.
  • CSC-C0425
  • Description
  • Established from the tumor specimen resected from the urinary bladder transitional cell carcinoma (undifferentiated, grade IV) of a 67-year-old woman in 1974 (without therapy)
  • Recommended Medium
  • 80-85% DMEM (4.5 g/L glucose) + 15-20% h.i. FBS
  • Organism
  • Human
  • Morphology
  • epithelial-like adherent cells with smooth borders growing as monolayer
  • Culture Properties
  • monolayer
  • Karyotype
  • Human hypotriploid karyotype with 8% polyploidy - 67(58-67)<3n>XX, -X, +1, -2, -4, +5, -9, -12, -18, -21, +1-2mar, der(1;21)(q10;q10), del(1)(q13), i(5p)x3, del(6)(q14), add(6)(p23)/der(6)t(3;6)(p22;p22), add(6)(p22/23), del(10)(p11), add(20)(q12), add(21
  • Quality Control
  • Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays
    Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, cytokeratin-19
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • urinary bladder transitional cell carcinoma

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Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

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Tel: 44-207-048-3343
Email: info@creative-bioarray.com