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  • Cat.No.
  • CSC-C2512
  • Description
  • Established from pleural effusion of a ductal carcinoma of the breast of a 54-year-old female. The differentiated epithelial sub-strain T-47D reportedly contains cytoplasmic junctions, receptors to 17-beta-estradiol, other steroids and calcitonin. It will form colonies in soft agar. The cells express the WNT7B oncogene.
  • Recommended Medium
  • RPMI 1640 medium supplemented with 4.5g/L glucose, 2 mM L-glutamine, and 10% FBS
  • Organism
  • Homo sapiens, human
  • Morphology
  • Epithelial
  • STR DNA Profile
  • Amelogenin: X,X
    CSF1PO:11,13
    D13S317:12
    D16S539:10
    D5S818:12
    D7S820:11
    TH01:7,6
    TPOX:11
    vWA:14
    D3S1358: 15,17
    D21S11: 28,31
    D18S51: 17
    Penta E: 7,14
    Penta D: 10,12
    D8S1179: 13
    FGA: 23
  • Culture Properties
  • monolayer
  • Karyotype
  • This is a hypotriploid human cell line. The modal chromosome number is 65 occurring at 50% and polyploidy at 0.8%. 18 marker chromosomes are common to most cells, of which 7 are paired and 11 are single-copied. The t(8q14q), t(9q17q), t(10q17p) are among 7 paired markers common to most cells. N7, N9, and N10 are absent and N11 is generally present in 4 copies. DM's occurred, but infrequently. Q-band examination did not show the presence of a Y chromosome.
  • Source
  • Breast; mammary gland
  • Quality Control
  • Sterility: mycoplasma contamination eliminated with BM-cyclin; negative, HOECHST and PCR
  • Storage and Shipping
  • Frozen with Culture medium + 50% FBS + 10% DMSO; ship in dry ice; store in liquid nitrogen
  • Tissue
  • Breast; mammary gland
  • Application
  • This cell line can be used as a suitable transfection host.
  • Disease
  • Invasive ductal carcinoma

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Email: info@creative-bioarray.com