Established from the primary tumor of an 81-year-old Caucasian woman with urinary bladder carcinoma (transitional cell carcinoma, grade III) in 1970; described to produce a variety of cytokines (e.g. G-CSF, IL-6 and SCF) and to carry a p53 mutation. Leukocytes and sera from patients with transitional cell carcinoma were cytotoxic to T24 and related lines. T24 cells have a generation time of 19 hours.
DMEM:Ham's F12 medium (1:1 mixture) supplemented with 2 mM L-glutamine, 15 mM Hepes and 5% fetal bovine serum.
hypodiploidy to hypopentaploidy; stemline 86; 2 to 4 telocentrics; 3 to 4 minutes, hypotetraploid to hypertetraploid with abnormalities including dicentrics, breaks, pulverization, minutes and telocentric markers.
Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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