- Established from the primary tumor of an 81-year-old Caucasian woman with urinary bladder carcinoma (transitional cell carcinoma, grade III) in 1970; described to produce a variety of cytokines (e.g. G-CSF, IL-6 and SCF) and to carry a p53 mutation. Leukocytes and sera from patients with transitional cell carcinoma were cytotoxic to T24 and related lines. T24 cells have a generation time of 19 hours.
- Homo sapiens ,human
- STR DNA Profile
- Amelogenin: X,X
Penta E: 7,10
Penta D: 11,15
- Culture Properties
- hypodiploidy to hypopentaploidy; stemline 86; 2 to 4 telocentrics; 3 to 4 minutes, hypotetraploid to hypertetraploid with abnormalities including dicentrics, breaks, pulverization, minutes and telocentric markers.
- Quality Control
- Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -,
- Storage and Shipping
- Frozen with 70% medium, 20% FBS, 10% DMSO at about 2.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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