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T24;T-24

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  • Cat.No.
  • CSC-C0424
  • Description
  • Established from the primary tumor of an 81-year-old Caucasian woman with urinary bladder carcinoma (transitional cell carcinoma, grade III) in 1970; described to produce a variety of cytokines (e.g. G-CSF, IL-6 and SCF) and to carry a p53 mutation. Leukocytes and sera from patients with transitional cell carcinoma were cytotoxic to T24 and related lines. T24 cells have a generation time of 19 hours.
  • Recommended Medium
  • DMEM:Ham's F12 medium (1:1 mixture) supplemented with 2 mM L-glutamine, 15 mM Hepes and 5% fetal bovine serum.
  • Organism
  • Homo sapiens ,human
  • Morphology
  • epithelial
  • STR DNA Profile
  • Amelogenin: X,X
    CSF1PO: 10,12
    D13S317: 12,12
    D16S539: 9,9
    D5S818: 10,12
    D7S820: 10,11
    THO1: 6,6
    TPOX: 8,11
    vWA: 17,19
    D3S1358: 16,21
    D21S11: 29,29
    D18S51: 16,18
    Penta E: 7,10
    Penta D: 11,15
    D8S1179: 9,14
    FGA: 17,22
  • Culture Properties
  • monolayer
  • Karyotype
  • hypodiploidy to hypopentaploidy; stemline 86; 2 to 4 telocentrics; 3 to 4 minutes, hypotetraploid to hypertetraploid with abnormalities including dicentrics, breaks, pulverization, minutes and telocentric markers.
  • Source
  • bladder, urinary
  • Quality Control
  • Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
    Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -,
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 2.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • bladder, urinary

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Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com