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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-C0257
  • Description
  • Established from a lung metastasis of colon carcinoma in a 72 year old male. Tumour tissue was inoculated sub-cutaneously and serially transplanted in BALB/c nude mice and subsequently established in in vitro culture. The histological characteristics of the tumour were maintained throughout the transplantation procedure. They have receptors for a range of peptide hormones and neurotransmitters. The cells grow as monolayers, exhibit tight junctions and desmosomes between adjacent cells. The cells should be maintained at high density (at least 1/4 confluency) and are positive for keratin by immunoperoxidase staining.
  • Organism
  • Homo sapiens, human
  • Morphology
  • epithelial
  • STR DNA Profile
  • Amelogenin: X
    CSF1PO: 10
    D13S317: 9
    D16S539: 10,11
    D5S818: 12
    D7S820: 8,10
    THO1: 6,9
    TPOX: 8
    vWA: 17,18
    D3S1358: 19
    D21S11: 31
    D18S51: 17
    Penta E: 14
    Penta D: 9
    D8S1179: 15
    FGA: 24
  • Culture Properties
  • Adherent
  • Karyotype
  • The stemline modal chromosome number is 56, occurring at 28% with polyploidy at 12.4%. Eighteen markers are common to most metaphases examined. Normal X and chromosome 13 were absent; chromosomes 2, 4 and 22 were single-copied, and chromosome 12 was 4-copied. No Y chromosome was detected by Q band observation. DM occurred in nearly 50% of the cells.
  • Source
  • Carcinoma, colorectal; Colon; from metastatic site: lung
  • Quality Control
  • Tests for mycoplasma, bacteria and fungi were negative
  • Storage and Shipping
  • liquid nitrogen vapor phase
  • Application
  • The T84 cell line can be used for tumourigenicity, neurotransmitter and hormone studies. It is a transplantable human carcinoma cell line and also can be used as a suitable transfection host.

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    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com