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SW-480

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-C0256
  • Description
  • Established from the tumor of a 50-year-old Caucasian man with colon adenocarcinoma (grade 4, Duke class B). The cells express elevated levels of p53 protein. The cells have been reported to produce GM-CSF. SW-480 were described to produce carcinoembryonic antigen (CEA) 0.7 ng/10 6 cells/10 days; keratin; transforming growth factor beta, myc +; myb +; ras +; fos +; sis +; p53 +; abl -; ros -; src -, HLA A2, B8, B17; blood type A; Rh+. The cells are positive for keratin by immunoperoxidase staining and are positive for expression of c-myc, K-ras, H-ras, N-ras, myb, sis and fos oncogenes.
  • Recommended Medium
  • SuperCult® SW-480 Medium
  • Organism
  • Homo sapiens, human
  • Morphology
  • Epithelial
  • STR DNA Profile
  • Amelogenin: X,X
    CSF1PO: 13,14
    D13S317: 12
    D16S539: 13
    D5S818: 13
    D7S820: 8
    THO1: 8
    TPOX: 11
    vWA: 16
    D3S1358: 15
    D21S11: 30,30.2
    D18S51: 13
    Penta E: 10
    Penta D: 9,15
    D8S1179: 13
    FGA: 24
  • Culture Properties
  • monolayer, adherent
  • Karyotype
  • The stemline chromosome number is hypotriploid and 11-12 marker chromosomes were common. Both double minutes and dicentrics were observed in 8% of each metaphase examined.
  • Source
  • Colon
  • Quality Control
  • Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
    Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Application
  • The SW-480 cell line can be used as a suitable transfection host. This cell line has a mutation in codon 12 of the ras proto-oncogene, and can be used as a positive control for PCR assays of mutation in this codon.
  • Disease
  • Dukes' type B, colorectal adenocarcinoma

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    • USA
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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com